Create a new FlowObject — flowObject • GatingTree

This function constructs a new FlowObject using various inputs related to flow cytometry analysis, including data, sample definitions, metadata, and data processing configurations.

Usage

flowObject(
  Data = data.frame(),
  sampledef = new("SampleDef"),
  metadata = list(),
  prep = list(),
  Clustering = list(),
  Gating = list(),
  QCdata = list(),
  Transformation = list()
)

Arguments

Data: A data frame containing flow cytometric expression data.
sampledef: An object of class SampleDef, specifying sample definitions.
metadata: A list containing annotation data for the flow cytometry experiment.
prep: A list of character string vectors defining the samples and controls.
Clustering: A list containing cell identities and clustering data.
Gating: A list containing outputs of gating, including GatingTree objects.
QCdata: A list containing the quality control information for the flow cytometry data.
Transformation: A list containing settings for data transformation, including the log data settings.

Value

An object of class FlowObject, which encapsulates all provided data and settings for flow cytometry analysis.