Projects cells onto a piecewise Slerp manifold defined by the landmarks B -> BR -> R.
Automatically extracts the landmark vectors from the provided mCanonicalTockyObj.
Cells with negative correlations to all three landmarks are classified as "Timer Negative" (NA),
as they lie in the ordination space opposite to the Tocky trajectory.
Usage
mGradientTockySeq(object, ...)
# S4 method for class 'mCanonicalTockyObj'
mGradientTockySeq(object, filter_negative = TRUE, intensity_q = 0.75)Arguments
- object
An object of class
mCanonicalTockyObj.- ...
Additional arguments.
- filter_negative
Logical. If TRUE, assigns NA to cells with negative projection to all three landmarks.
- intensity_q
Numeric. Quantile threshold (0 to 1) for normalized intensity. Cells falling below this threshold are treated as non-participating and assigned NA for angle. Default is 0.75.