Masahiro Ono

MD PhD FHEA

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Dr Masahiro Ono

Department of Life Sciences

Imperial College London

London SW7 2AZ

Masahiro Ono is an Integrative Experimental and Computational Immunologist. He is known as the creator and developer of the innovative Timer-of-Cell-Kinetics-and-Activity (Tocky) technology.

Expertise:

  • Integrative Experimental and Computational Immunology
  • Molecular and Systems Immunology
  • Cancer Immunology
  • Computational Biology for Cytometry and Genomics

Innovations and Tools:

  • Developer of Tocky (とき)
  • Application of Canonical Correspondence Analysis in Genomic Analysis

Current Position:

Education:

  • Kyoto University, Japan

news

Nov 06, 2024 💻 New R Package ReleasedTockyPrep: We have released the TockyPrep package, which offers data preprocessing methods for Tocky data (and any Fluorescent Timer data). Such data would not be fully useful without these proper analytic methods. Our preprint is accessible here
Nov 05, 2024 💻 New R Package ReleasedGatingTree: A ground-breaking R package for analysing high-dimensional cytometry data without the use of dimensional reduction. 🚀 See the GatingTree website and my research paper for the details!
Jun 13, 2024 📄 New Paper Published: The spectrum of self-reactive T cells and their activation mechanisms exploited by HTLV-1 infection 🔬🧬
Mar 25, 2024 🚀 A new website has launched! 🌐✨

latest posts

selected publications

  1. A temporally dynamic Foxp3 autoregulatory transcriptional circuit controls the effector Treg programme
    David Bending , Alina Paduraru , Catherine B Ducker , Paz Prieto Martı́n , Tessa Crompton , and Masahiro Ono
    The EMBO journal, 2018
    The second Tocky paper from the Ono lab uncovers the temporally dynamic regulation of Foxp3 transcription, offering new insights into T cell regulation.
  2. A timer for analyzing temporally dynamic changes in transcription during differentiation in vivo
    David Bending , Paz Prieto Martı́n , Alina Paduraru , Catherine Ducker , Erik Marzaganov , Marie Laviron , Satsuki Kitano , Hitoshi Miyachi , Tessa Crompton , and Masahiro Ono
    Journal of Cell Biology, 2018
    The foundational publication introducing Tocky technology by the Ono lab, marking a breakthrough in T cell and B cell studies.
  3. Controversies concerning thymus‐derived regulatory T cells: fundamental issues and a new perspective
    Masahiro Ono, and Reiko J Tanaka
    Immunology and cell biology, 2016
    A landmark opinion piece challenging the reproducibility of a foundational Treg experiment, while introducing a groundbreaking dynamic view on Foxp3-mediated T cell regulation.
  4. Regulatory T cells in melanoma revisited by a computational clustering of FOXP3+ T cell subpopulations
    Hiroko Fujii , Julie Josse , Miki Tanioka , Yoshiki Miyachi , François Husson , and Masahiro Ono
    The Journal of Immunology, 2016
    The pioneering study ntroduces a data-driven methodology to explore Treg subpopulations, employing Principal Component Analysis (PCA) for the first time.
  5. Visualisation of the T cell differentiation programme by Canonical Correspondence Analysis of transcriptomes
    Masahiro Ono, Reiko Tanaka , and Manabu Kano
    BMC Genomics, 2014
    This study establishes CCA as a vital quantitative tool for transcriptome analysis in immunological datasets.
  6. Visualising the cross-level relationships between pathological and physiological processes and gene expression: analyses of haematological diseases
    Masahiro Ono, Reiko Tanaka , Manabu Kano , and Toshio Sugiman
    PLoS One, 2013
    The pioneering study developing Canonical Correspondence Analysis (CCA) as a genomics method, establishing a novel approach for transcriptome analysis.