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📄 New Preprint Published 🚀 Our new bioRxiv preprint introduces CanonicalTockySeq, a framework that integrates Nr4a3-Tocky Fluorescent Timer signalling history with single-cell RNA-seq to resolve dynamic T-cell states in cancer immunotherapy.

Using an experimentally anchored canonical manifold, this study separates temporal progression from signalling strength at single-cell resolution and shows that effective checkpoint blockade is associated with reduced persistence of antigen engagement, suppression of exhaustion-associated TCR signalling programmes, and maintenance of progenitor-like features linked to durable antitumour responses.

• Hassan J, Reda O, Irie N, Pedersen M, Foo S, Appleton L, Okazaki I, Okazaki T, Satou Y, Harrington K, Melcher A, Ono M (2026). Temporal Mechanisms of T-Cell Fate Decisions under Immune Checkpoint Blockade Resolved by CanonicalTockySeq. bioRxiv. https://doi.org/10.64898/2026.03.10.710825 🔬🧬