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📄 New Paper Published 🕒 Our paper in Biology Methods and Protocols introduces TockyLocus, an open-source framework for the quantitative and standardised analysis of Fluorescent Timer flow cytometry data in Nr4a3-Tocky and Foxp3-Tocky mice.
By optimising analysis of Timer Angle and establishing a biologically grounded five-locus categorisation scheme, this study provides a robust and interpretable method for analysing transcriptional dynamics, enabling reproducible statistical testing and visualisation without reliance on arbitrary gating.
- Ono M (2025). TockyLocus: quantitative analysis of flow cytometric fluorescent timer data in Nr4a3-Tocky and Foxp3-Tocky mice. Biology Methods and Protocols 10(1): bpaf060. https://doi.org/10.1093/biomethods/bpaf060 🔬🧬